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Dr. Bruce M. Applegate

Associate Professor

B.A., Ph.D., University of Tennessee,

Research

Detection of viable foodborne pathogens using bateriophage; automated extraction of nucleic acids from various matrices; enumeration of microorganisms (i.e. pathogens and other organisms) using quantitative PCR; the use of bioreporters in bioelectronics; metabolic engineering; detection of problematic microorganisms in industrial environments; construction of recombinant bacterial strains to rapidly evaluate antimicrobial products; microbial ecology.

Selected Publications

Simpson, M.L., G.S. Sayler, G. Patterson, D.E. Nivens, E.K. Bolton, J.M. Rochelle, J.C. Arnott, B.M. Applegate, S. Ripp and M.A. Guillorn. 2001. An integrated CMOS microluminometer for low-level luminescence sensing in the bioluminescent bioreporter integrated circuit. Sensors and Actuators B. 72:134-140.

Hay, A.G., J.F. Rice, B.M. Applegate, N.G. Bright, and G.S. Sayler. 2000. A Bioluminescent Whole-Cell Reporter for Detection of 2,4-Dichlorophenoxyacetic Acid and 2,4-Dichlorophenol in Soil. Applied and Environmental Microbiology. Vol. 66, No. 10, p. 4589-4594.

LeBlond, J.D., B.M. Applegate, F.-M. Menn, T.W. Schultz, and G.S. Sayler. 2000. Structure-toxicity assessment of metabolites of the aerobic bacterial transformation of substituted naphthalenes. Environmental Toxicology and Chemistry. Vol. 19, No. 5, p. 1235-1246.

Ripp, S., B.M. Applegate, D.E. Nivens, M.J. Paulus, G.E. Jellison, M.L. Simpson, and G.S. Sayler. 2000. Whole-Cell Environmental Monitoring Devices: Bioluminescent Bioreporter Integrated Circuits. (Mulchandi and Sadik, eds.) In: ACS Symposium Series No. 762/Chemical and Biological Sensors for Environmental Monitoring. p. 197-205.

Simpson, M. L., Michael J. Paulus, G. E. Jellison, G. S. Sayler, B. M. Applegate, S. Ripp, and D. E. Nivens.1999. “Bioluminescent Bioreporter Integrated Circuits (BBICs): Whole-Cell Environmental Monitoring Devices”. Proceedings of the 29th International Conference on Environmental Systems (Society of Automotive Engineers), Denver, CO.

Sayler, G. S., S. Ripp, D. E. Nivens, B. M. Applegate, and M. L. Simpson. "Bioluminescent Bioreporters: Moving Toward Smart Systems in Bioremediation Process Monitoring and Control". Proceedings of the 1st Arab Environmental Biotechnology Conference, Abu Dhabi, March 1999.

Applegate, B.M., J. Shingleton, S. Ripp, N. Bright, D. Nivens, M. Simpson, and G.S. Sayler. 1999. Bioluminescent Bioreporter Integrated Circuits (BBICs) for the Detection of Toluene and Trichloroethylene (TCE) in Aqueous Solutions. In: Bioluminescence and Chemiluminescence: Perspectives for the 21st Century, eds. A. Roda, M. Pazzagli, L.J. Kricka and P.E. Stanley. p. 589-592.

Palmer, R.J., B.M. Applegate, R. Burlage, G.S. Sayler and D.C. White. 1999. Heterogeneity of Gene Expression and Activity in Bacterial Biofilms. In: Bioluminescence and Chemiluminescence: Perspectives for the 21st Century, eds. A. Roda, M. Pazzagli, L.J. Kricka and P.E. Stanley. p.609-612.

Shingleton, J.T., B.M. Applegate, A.C. Nagel, P.R. Bienkowski, and G.S. Sayler. 1998. Induction of the tod Operon by trichloroethylene in Pseudomonas putida TVA8. Applied and Environmental Microbiology. 64: 5049-5052.

Simpson, M.L., G.S. Sayler, B.M. Applegate, S. Ripp, D.E. Nivens, M.J. Paulus, and G. E. Jellison Jr. 1998. Bioluminescent bioreporter integrated circuits: novel whole-cell biosensors. Trends in Biotechnology. 16: 332-338.

Contact Dr. Applegate

e-mail: applegate@purdue.edu

Address:
Department of Food Science
745 Agriculture Mall Drive
West Lafayette, IN 47907-2009

Office: Food Science Building Room 3161

Phone: (765) 496-7920